陈 娟^1，2，康文丽^2，3*，侯艳梅²，汪家琦^2，3，潘丽娜^2，3，李 威²，王远亮^1*

（1.湖南农业大学食品科学技术学院，长沙410125；2.澳优乳业（中国）有限公司，长沙410000；3.人体微生态制品湖南省工程研究中心，长沙410017）

摘要：以植物乳植杆菌（Lactiplantibacillus plantarum）M660为研究对象，进行冻干保护剂筛选和菌粉的特性研究。采用单因素及响应面试验得到冻干保护剂配方，通过模拟体外消化试验和在－20℃、4℃、25℃储藏稳定性试验验证冻干保护剂的效果。结果表明：保护剂的配方为低聚木糖9.5%、全脂奶粉9.5%、谷氨酸钠6%，在此条件下菌株存活率为88.39%；菌粉在模拟口腔、模拟胃液和模拟肠液中的存活率分别为91.9%、41.9%、18.4%；在－20℃储藏28d菌株存活率为82.5%。

关键词：植物乳植杆菌；冻干保护剂；响应面组合试验；模拟体外消化；储藏稳定性

中图分类号：TS201.3 文献标识码：A 文章编号：1674-506X（2025）02-0092-0006

Screening of Lactiplantibacillus plantarum Lyophilization Protectants and Research on the Property of Bacterial Powder

CHEN Juan^1,2, KANG Wenli^2,3*, HOU Yanmei², WANG Jiaqi^2,3, PAN Lina^2,3, LI Wei², WANG Yuanliang^1*

(1.School of Food Science and Technology, Hunan Agricultural University, Changsha 410125, China;

2.Ausnutria Dairy(China) Co., Ltd., Changsha 410000, China;

3.Hunan Engineering Research Center of Human Microecological Products, Changsha 410017, China)

Abstract: Lactiplantibacillus plantarum M660 was used as the research object for lyophilization protectants screening and bacterial powder property research. The lyophilization protectants formulation was obtained by single factor and response surface tests, and the effect of the screened lyophilization protectants was verified by simulated in vitro digestion tests and storage stability tests at -20 ℃,4 ℃ and 25 ℃. The results showed that: the formula of the protectant was 9.5% xylo-oligosaccharides, 9.5% whole milk powder and 6% monosodium glutamate, and under these conditions the survival rate of the bacterial powder was about 88.39%; the survival rates of the bacterial powder in simulated oral cavity, simulated gastric fluid and simulated intestinal fluid were 91.9%,41.9% and 18.4%; and the survival rate of the bacterial powder in storage at -20 ℃ for 28 d was 82.5%.

Keyword: Lactiplantibacillus plantarum; lyophilization protectants; response surface composite experiment; simulated in vitro digestion; storage stability

doi: 10.3969/j.issn.1674-506X.2025.02-012